› 8wc8

Electron Microscopy
2.9Å resolution

Cryo-EM structure of the ZH8651-bound hTAAR1-Gs complex

Released:
DOI: 10.2210/pdb8wc8/pdb
PDB entry 8wc8 coloured by chain, front view.
PDB entry 8wc8 coloured by chain, side view.
PDB entry 8wc8 coloured by chain, top view.
Source organisms:
Primary publication:
Structural and signaling mechanisms of TAAR1 enabled preferential agonist design.
Shang P, Rong N, Jiang JJ, Cheng J, Zhang MH, Kang D, Qi L, Guo L, Yang GM, Liu Q, Zhou Z, Li XB, Zhu KK, Meng QB, Han X, Yan W, Kong Y, Yang L, Wang X, Lei D, Feng X, Liu X, Yu X, Wang Y, Li Q, Shao ZH, Yang F, Sun JP
Cell 186 5347-5362.e24 (2023)
PMID: 37963465
Related structures: EMD-37434

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-273948 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 362 amino acids
Theoretical weight: 42.01 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-3, 27-67, 205-251, 266-394; Coverage: 56%)
  • Canonical: P63096 (Residues: 4-19, 61-181, 229-242; Coverage: 40%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Trace amine-associated receptor 1 Chain: R
Molecule details ›
Chain: R
Length: 339 amino acids
Theoretical weight: 39.13 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q96RJ0 (Residues: 1-339; Coverage: 100%)
Gene names: TA1, TAAR1, TAR1, TRAR1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 285 amino acids
Theoretical weight: 30.36 KDa
Source organism: synthetic construct
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: Y
Molecule details ›
Chain: Y
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

1 bound ligand:
Ligand VMT 1 x VMT
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-37434
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

The role of the gut microbiota in neurodegenerative diseases targeting metabolism.
Fu et al. (2024)
8 other articles

2 mentions without citation

Computational Methods for the Discovery and Optimization of TAAR1 and TAAR5 Ligands.
Scarano et al. (2024)
1 more