› 8wcq

Electron Microscopy
3.35Å resolution

Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in intermediate state

Released:
DOI: 10.2210/pdb8wcq/pdb
PDB entry 8wcq coloured by chain, front view.
PDB entry 8wcq coloured by chain, side view.
PDB entry 8wcq coloured by chain, top view.
Source organism: Gloeobacter violaceus
Primary publication:
Cryo-EM structures of prokaryotic ligand-gated ion channel GLIC provide insights into gating in a lipid environment.
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun 15 2967 (2024)
PMID: 38580666
Related structures: EMD-37446

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton-gated ion channel Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 312 amino acids
Theoretical weight: 35.7 KDa
Source organism: Gloeobacter violaceus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NDN8 (Residues: 47-358; Coverage: 99%)
Gene names: glr4197, glvI
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain

Ligands and Environments

2 bound ligands:
Ligand CL 5 x CL
Ligand PEE 40 x PEE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.35Å
Relevant EMDB volumes: EMD-37446
Expression system: Escherichia coli

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