› 8wg7

Electron Microscopy
2.54Å resolution

Cryo-EM structures of peptide free and Gs-coupled GLP-1R

Released:
DOI: 10.2210/pdb8wg7/pdb
PDB entry 8wg7 coloured by chain, front view.
PDB entry 8wg7 coloured by chain, side view.
PDB entry 8wg7 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular features of the ligand-free GLP-1R, GCGR and GIPR in complex with Gs proteins.
Cong Z, Zhao F, Li Y, Luo G, Mai Y, Chen X, Chen Y, Lin S, Cai X, Zhou Q, Yang D, Wang MW
OpenAccess logo Cell Discov 10 18 (2024)
PMID: 38346960
Related structures: EMD-37504

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-240851 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 1-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 371 amino acids
Theoretical weight: 40.23 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody-35 Chain: N
Molecule details ›
Chain: N
Length: 128 amino acids
Theoretical weight: 13.89 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Glucagon-like peptide 1 receptor Chain: R
Molecule details ›
Chain: R
Length: 440 amino acids
Theoretical weight: 50.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43220 (Residues: 24-463; Coverage: 100%)
Gene name: GLP1R
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.54Å
Relevant EMDB volumes: EMD-37504
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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