8wjn

Electron Microscopy
5.58Å resolution

Cryo-EM structure of 6-subunit Smc5/6 head region

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-274388 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Structural maintenance of chromosomes protein 6 Chain: B
Molecule details ›
Chain: B
Length: 1114 amino acids
Theoretical weight: 128.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
Structural maintenance of chromosomes protein 5 Chain: A
Molecule details ›
Chain: A
Length: 1093 amino acids
Theoretical weight: 126.24 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
Non-structural maintenance of chromosomes element 1 Chain: F
Non-structural maintenance of chromosome element 3 Chain: G
Non-structural maintenance of chromosomes element 4 Chain: H

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.58Å
Relevant EMDB volumes: EMD-37586
Expression system: Spodoptera frugiperda