› 8wjo

Electron Microscopy
6.04Å resolution

Cryo-EM structure of 8-subunit Smc5/6 arm region

Released:
DOI: 10.2210/pdb8wjo/pdb
PDB entry 8wjo coloured by chain, front view.
PDB entry 8wjo coloured by chain, side view.
PDB entry 8wjo coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cryo-EM structures of Smc5/6 in multiple states reveal its assembly and functional mechanisms.
Li Q, Zhang J, Haluska C, Zhang X, Wang L, Liu G, Wang Z, Jin D, Cheng T, Wang H, Tian Y, Wang X, Sun L, Zhao X, Chen Z, Wang L
Nat Struct Mol Biol (2024)
PMID: 38890552
Related structures: EMD-37587

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-246367 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Structural maintenance of chromosomes protein 5 Chain: A
Molecule details ›
Chain: A
Length: 1093 amino acids
Theoretical weight: 126.24 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q08204 (Residues: 1-1093; Coverage: 100%)
Gene names: SMC5, YOL034W
Sequence domains: RecF/RecN/SMC N terminal domain
Structural maintenance of chromosomes protein 6 Chain: B
Molecule details ›
Chain: B
Length: 1114 amino acids
Theoretical weight: 128.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q12749 (Residues: 1-1114; Coverage: 100%)
Gene names: L3502.2, RHC18, SMC6, YLR383W
Sequence domains: AAA domain
E3 SUMO-protein ligase MMS21 Chain: C
Molecule details ›
Chain: C
Length: 267 amino acids
Theoretical weight: 30.39 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P38632 (Residues: 1-267; Coverage: 100%)
Gene names: MMS21, NSE2, YEL019C
Sequence domains:
DNA repair protein KRE29 Chain: D
Molecule details ›
Chain: D
Length: 464 amino acids
Theoretical weight: 53.84 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P40026 (Residues: 1-464; Coverage: 100%)
Gene names: KRE29, YER038C
Sequence domains: DNA repair proteins Nse5 and Nse6

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.04Å
Relevant EMDB volumes: EMD-37587
Expression system: Spodoptera frugiperda

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