› 8wlo

Electron Microscopy
2.62Å resolution

Cryo-EM structure of SARS-CoV-2 prototype spike protein in complex with hippopotamus ACE2

Released:
DOI: 10.2210/pdb8wlo/pdb
PDB entry 8wlo coloured by chain, front view.
PDB entry 8wlo coloured by chain, side view.
PDB entry 8wlo coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of hippopotamus ACE2 binding to SARS-CoV-2.
Yang R, Han P, Han P, Li D, Zhao R, Niu S, Liu K, Li S, Tian W-X, Gao GF
J Virol 98 e0045124 (2024)
PMID: 38591877
Related structures: EMD-37626

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-242289 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Spike glycoprotein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1217 amino acids
Theoretical weight: 135.12 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 1-1217; Coverage: 96%)
Gene names: 2, S
Sequence domains:
Angiotensin-converting enzyme Chain: E
Molecule details ›
Chain: E
Length: 597 amino acids
Theoretical weight: 69.34 KDa
Source organism: Hippopotamus amphibius
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 25 x NAG
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.62Å
Relevant EMDB volumes: EMD-37626
Expression systems:
  • Homo sapiens
  • Escherichia coli

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