› 8wm8

Electron Microscopy
3.54Å resolution

Cryo-EM structure of cyanobacterial nitrate/nitrite transporter NrtBCD in complex with nitrate

Released:
DOI: 10.2210/pdb8wm8/pdb
PDB entry 8wm8 coloured by chain, front view.
PDB entry 8wm8 coloured by chain, side view.
PDB entry 8wm8 coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Primary publication:
Allosteric regulation of nitrate transporter NRT via the signaling protein PII.
Li B, Wang XQ, Li QY, Xu D, Li J, Hou WT, Chen Y, Jiang YL, Zhou CZ
Proc Natl Acad Sci U S A 121 e2318320121 (2024)
PMID: 38457518
Related structures: EMD-37645

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-240551 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ABC transporter domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 277 amino acids
Theoretical weight: 31.4 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8YZ75 (Residues: 1-277; Coverage: 100%)
Gene name: nrtD
Sequence domains: ABC transporter
ABC transmembrane type-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 279 amino acids
Theoretical weight: 30.48 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8YZ77 (Residues: 1-279; Coverage: 100%)
Gene name: nrtB
Sequence domains: Binding-protein-dependent transport system inner membrane component
ABC transporter domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 682 amino acids
Theoretical weight: 76.33 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8YZ76 (Residues: 1-657; Coverage: 100%)
Gene name: nrtC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.54Å
Relevant EMDB volumes: EMD-37645
Expression system: Escherichia coli BL21(DE3)

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