› 8wmc

Electron Microscopy
2.9Å resolution

Cryo-EM structure of DiCas7-11-crRNA in complex with regulator

Released:
DOI: 10.2210/pdb8wmc/pdb
PDB entry 8wmc coloured by chain, front view.
PDB entry 8wmc coloured by chain, side view.
PDB entry 8wmc coloured by chain, top view.
Source organism: Desulfonema ishimotonii
Primary publication:
Structural basis of negative regulation of CRISPR-Cas7-11 by TPR-CHAT.
Hong T, Luo Q, Ma H, Wang X, Li X, Shen C, Pang J, Wang Y, Chen Y, Zhang C, Su Z, Dong H, Tang X
OpenAccess logo Signal Transduct Target Ther 9 111 (2024)
PMID: 38735995
Related structures: EMD-37649

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244699 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
CHAT domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 751 amino acids
Theoretical weight: 88.31 KDa
Source organism: Desulfonema ishimotonii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A401FT52 (Residues: 1-751; Coverage: 100%)
Gene name: DENIS_1081
Sequence domains: CHAT domain
CRISPR-associated RAMP family protein Chain: A
Molecule details ›
Chain: A
Length: 1601 amino acids
Theoretical weight: 183.93 KDa
Source organism: Desulfonema ishimotonii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A401FT36 (Residues: 1-1601; Coverage: 100%)
Gene name: DENIS_1082
Sequence domains: RAMP superfamily
RNA (38-MER) Chain: R
Molecule details ›
Chain: R
Length: 38 nucleotides
Theoretical weight: 12.13 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-37649
Expression system: Escherichia coli

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