› 8wnj

X-ray diffraction
1.78Å resolution

Crystal structure of H. pylori isoleucyl-tRNA synthetase (HpIleRS) in complex with Ile-AMP

Released:
DOI: 10.2210/pdb8wnj/pdb
PDB entry 8wnj coloured by chain, front view.
PDB entry 8wnj coloured by chain, side view.
PDB entry 8wnj coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Structural basis for substrate and antibiotic recognition by Helicobacter pylori isoleucyl-tRNA synthetase.
Chen X, Guo Y, Shi J, Wang Y, Guo X, Wu G, Li S, Zhang T
FEBS Lett (2024)
PMID: 38246751

Function and Biology Details

Reaction catalysed: 
ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-547785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isoleucine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 920 amino acids
Theoretical weight: 106.23 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: P56456 (Residues: 1-920; Coverage: 100%)
Gene names: HP_1422, ileS
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand GOL 3 x GOL
Ligand GV6 1 x GV6
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 52.783Å b: 52.912Å c: 103.054Å
α: 92.41° β: 97.37° γ: 104.35°
R-values:
R R work R free
0.187 0.185 0.217
Expression system: Escherichia coli

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