› 8wov

X-ray diffraction
2.25Å resolution

Crystal structure of Arabidopsis thaliana UDP-glucose 4-epimerase 2 (AtUGE2) complexed with UDP, G233A mutant

Released:
DOI: 10.2210/pdb8wov/pdb
PDB entry 8wov coloured by chain, front view.
PDB entry 8wov coloured by chain, side view.
PDB entry 8wov coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Cytosolic UDP-L-arabinose synthesis by bifunctional UDP-glucose 4-epimerases in Arabidopsis.
Umezawa A, Matsumoto M, Handa H, Nakazawa K, Miyagawa M, Seifert GJ, Takahashi D, Fushinobu S, Kotake T
Plant J (2024)
PMID: 38678521

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 370 amino acids
Theoretical weight: 40.61 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9T0A7 (Residues: 1-350; Coverage: 100%)
Gene names: At4g23920, T32A16.90, UGE2
Sequence domains: GDP-mannose 4,6 dehydratase

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand UDP 2 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 101.49Å b: 109.243Å c: 130.627Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.168 0.228
Expression system: Escherichia coli BL21(DE3)

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