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Cryo-EM structure of CUL2-RBX1-ELOB-ELOC-FEM1B bound with the C-degron of CCDC89 (conformation 2)

Source organism: Homo sapiens
Biochemical function: protein binding
Biological process: protein ubiquitination
Cellular component: Cul2-RING ubiquitin ligase complex

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EC 2.3.2.32: Cullin-RING-type E3 NEDD8 transferase

Reaction catalysed:
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
Systematic name:
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine:[cullin] [NEDD8-protein] transferase (isopeptide bond-forming; RING-type)
Alternative Name(s):
  • RBX1 (gene name)

EC 2.3.2.27: RING-type E3 ubiquitin transferase

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Systematic name:
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine:[acceptor protein]-L-lysine ubiquitin transferase (isopeptide bond-forming; RING-type)
Alternative Name(s):
  • RING E3 ligase
  • Ubiquitin transferase RING E3

GO terms

Biochemical function:
Biological process:
Cellular component:

Sequence families

Pfam Protein families (Pfam)
PF12796
Domain description: Ankyrin repeats (3 copies)
Occurring in:
  1. Protein fem-1 homolog B
The deposited structure of PDB entry 8wqb contains 6 copies of Pfam domain PF12796 (Ankyrin repeats (3 copies)) in Protein fem-1 homolog B. Showing 3 copies in chain K [auth F].

PF12678
Domain description: RING-H2 zinc finger domain
Occurring in:
  1. E3 ubiquitin-protein ligase RBX1, N-terminally processed
The deposited structure of PDB entry 8wqb contains 2 copies of Pfam domain PF12678 (RING-H2 zinc finger domain) in E3 ubiquitin-protein ligase RBX1, N-terminally processed. Showing 1 copy in chain J [auth E].

PF00240
Domain description: Ubiquitin family
Occurring in:
  1. Elongin-B
The deposited structure of PDB entry 8wqb contains 2 copies of Pfam domain PF00240 (Ubiquitin family) in Elongin-B. Showing 1 copy in chain G [auth D].

PF03931
Domain description: Skp1 family, tetramerisation domain
Occurring in:
  1. Elongin-C
The deposited structure of PDB entry 8wqb contains 2 copies of Pfam domain PF03931 (Skp1 family, tetramerisation domain) in Elongin-C. Showing 1 copy in chain F [auth C].

PF10557
Domain description: Cullin protein neddylation domain
Occurring in:
  1. Cullin-2
The deposited structure of PDB entry 8wqb contains 2 copies of Pfam domain PF10557 (Cullin protein neddylation domain) in Cullin-2. Showing 1 copy in chain A.

PF00888
Domain description: Cullin family
Occurring in:
  1. Cullin-2
The deposited structure of PDB entry 8wqb contains 2 copies of Pfam domain PF00888 (Cullin family) in Cullin-2. Showing 1 copy in chain A.

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