› 8wst

Electron Microscopy
2.4Å resolution

Cryo-EM structure of Melanin-Concentrating Hormone Receptor 2 with MCH

Released:
DOI: 10.2210/pdb8wst/pdb
PDB entry 8wst coloured by chain, front view.
PDB entry 8wst coloured by chain, side view.
PDB entry 8wst coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanisms of ligand recognition and activation of melanin-concentrating hormone receptors.
He Q, Yuan Q, Shan H, Wu C, Gu Y, Wu K, Hu W, Zhang Y, He X, Xu HE, Zhao LH
Cell Discov 10 48 (2024)
PMID: 38710677
Related structures: EMD-37824

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-246046 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(q) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 349 amino acids
Theoretical weight: 38.34 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Melanin-concentrating hormone Chain: L
Molecule details ›
Chain: L
Length: 19 amino acids
Theoretical weight: 2.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P20382 (Residues: 147-165; Coverage: 13%)
Gene names: MCH, PMCH
NB35 Chain: N
Molecule details ›
Chain: N
Length: 131 amino acids
Theoretical weight: 14.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Melanin-concentrating hormone receptor 2 Chain: R
Molecule details ›
Chain: R
Length: 331 amino acids
Theoretical weight: 37.91 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q969V1 (Residues: 1-331; Coverage: 97%)
Gene names: GPR145, MCHR2, SLT
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.4Å
Relevant EMDB volumes: EMD-37824
Expression system: Spodoptera frugiperda

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