› 8wt4

X-ray diffraction
1.62Å resolution

Crystal structure of DL-endopeptidase CwlO complexed with IseA

Released:
DOI: 10.2210/pdb8wt4/pdb
PDB entry 8wt4 coloured by chain, front view.
PDB entry 8wt4 coloured by chain, side view.
PDB entry 8wt4 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structural analysis of the peptidoglycan DL-endopeptidase CwlO complexed with its inhibitory protein IseA.
Tandukar S, Kwon E, Kim DY
FEBS J (2024)
PMID: 38840475

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246578 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Uncharacterized protein YoeB Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 20.17 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34841 (Residues: 1-181; Coverage: 100%)
Gene names: BSU18380, yoeB
Sequence domains: IseA DL-endopeptidase inhibitor
Peptidoglycan DL-endopeptidase CwlO Chain: B
Molecule details ›
Chain: B
Length: 135 amino acids
Theoretical weight: 14.18 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40767 (Residues: 339-473; Coverage: 31%)
Gene names: BSU34800, cwlO, yvcE, yzkA
Sequence domains: NlpC/P60 family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 11C
Spacegroup: P21212
Unit cell:
a: 92.2Å b: 58.84Å c: 69.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.208
Expression system: Escherichia coli BL21(DE3)

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