8wut

Electron Microscopy
3Å resolution

SpCas9-MMLV RT-pegRNA-target DNA complex (initiation)

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-270246 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (6 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1367 amino acids
Theoretical weight: 158.46 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q99ZW2 (Residues: 2-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
Gag-Pol polyprotein Chain: E
Molecule details ›
Chain: E
Length: 496 amino acids
Theoretical weight: 55.66 KDa
Source organism: Moloney murine leukemia virus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8UN00 (Residues: 660-1155; Coverage: 29%)
Gene name: gag-pro-pol
Sequence domains:
DNA (51-MER) Chain: C
DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3') Chain: D
RNA (115-MER) Chain: B
DNA/RNA (35-MER) Chain: F

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-37859
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'