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SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 16-nt)

Electron Microscopy
3Å resolution
PDB entry 8wuv coloured by chain, front view.
PDB entry 8wuv coloured by chain, side view.
PDB entry 8wuv coloured by chain, top view.
Source organisms:
Assembly composition:
hetero hexamer (preferred)
Entry contents: 2 distinct polypeptide molecules
3 distinct DNA molecules
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CRISPR-associated endonuclease Cas9/Csn1 and MLV integrase and RNA and DNA
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Multimeric state: hetero hexamer
Accessible surface area: 110417.5 Å2
Buried surface area: 25308.46 Å2
Dissociation area: 1,173.29 Å2
Dissociation energy (ΔGdiss): 19.56 kcal/mol
Dissociation entropy (TΔSdiss): 11.14 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-245025

Macromolecules

CRISPR-associated endonuclease Cas9/Csn1
Chain: A
Length: 1367 amino acids
Theoretical weight: 158.46 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q99ZW2 (Residues: 2-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Pfam:
CRISPR-associated endonuclease Cas9/Csn1 in PDB entry 8wuv, assembly 1, front view.
CRISPR-associated endonuclease Cas9/Csn1 in PDB entry 8wuv, assembly 1, side view.
CRISPR-associated endonuclease Cas9/Csn1 in PDB entry 8wuv, assembly 1, top view.
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Gag-Pol polyprotein
Chain: E
Length: 496 amino acids
Theoretical weight: 55.66 KDa
Source organism: Moloney murine leukemia virus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8UN00 (Residues: 660-1155; Coverage: 29%)
Gene name: gag-pro-pol
Pfam:
Gag-Pol polyprotein in PDB entry 8wuv, assembly 1, front view.
Gag-Pol polyprotein in PDB entry 8wuv, assembly 1, side view.
Gag-Pol polyprotein in PDB entry 8wuv, assembly 1, top view.
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DNA chain C
Name: DNA (51-MER)
Representative chains: C
Length: 51 nucleotides
Theoretical weight: 15.64 KDa
DNA (51-MER) in PDB entry 8wuv, assembly 1, front view.
DNA (51-MER) in PDB entry 8wuv, assembly 1, side view.
DNA (51-MER) in PDB entry 8wuv, assembly 1, top view.
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DNA chain D
Name: DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3')
Representative chains: D
Length: 17 nucleotides
Theoretical weight: 5.29 KDa
DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3') in PDB entry 8wuv, assembly 1, front view.
DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3') in PDB entry 8wuv, assembly 1, side view.
DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3') in PDB entry 8wuv, assembly 1, top view.
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DNA chain F
Name: DNA (50-MER)
Representative chains: F
Length: 50 nucleotides
Theoretical weight: 15.44 KDa
DNA (50-MER) in PDB entry 8wuv, assembly 1, front view.
DNA (50-MER) in PDB entry 8wuv, assembly 1, side view.
DNA (50-MER) in PDB entry 8wuv, assembly 1, top view.
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RNA chain B
Name: RNA (137-MER)
Representative chains: B
Length: 137 nucleotides
Theoretical weight: 44.3 KDa
RNA (137-MER) in PDB entry 8wuv, assembly 1, front view.
RNA (137-MER) in PDB entry 8wuv, assembly 1, side view.
RNA (137-MER) in PDB entry 8wuv, assembly 1, top view.
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