› 8wvb

X-ray diffraction
2.5Å resolution

Crystal structure of Lsd18 mutant S195M

Released:
DOI: 10.2210/pdb8wvb/pdb
PDB entry 8wvb coloured by chain, front view.
PDB entry 8wvb coloured by chain, side view.
PDB entry 8wvb coloured by chain, top view.
Source organism: Streptomyces lasalocidi
Primary publication:
Simultaneous Improvement in the Thermostability and Catalytic Activity of Epoxidase Lsd18 for the Synthesis of Lasalocid A.
Liu N, Xiao H, Zang Y, Zhou L, Mencius J, Yang Z, Quan S, Chen X
OpenAccess logo Int J Mol Sci 24 (2023)
PMID: 38069118

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative epoxidase LasC Chains: A, B
Molecule details ›
Chains: A, B
Length: 488 amino acids
Theoretical weight: 52.69 KDa
Source organism: Streptomyces lasalocidi
Expression system: Escherichia coli
UniProt:
  • Canonical: B5M9L6 (Residues: 1-472; Coverage: 100%)
Gene names: lasC, lsd18
Sequence domains: FAD binding domain

Ligands and Environments

2 bound ligands:
Ligand FAD 2 x FAD
Ligand CL 2 x CL
1 modified residue:
Ligand ELY 2 x ELY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21
Unit cell:
a: 62.041Å b: 48.671Å c: 136.049Å
α: 90° β: 91.34° γ: 90°
R-values:
R R work R free
0.211 0.209 0.252
Expression system: Escherichia coli

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