› 8wvd

X-ray diffraction
1.66Å resolution

Crystal structure of Glycosyltransferase in complex with UD1

Released:
DOI: 10.2210/pdb8wvd/pdb
PDB entry 8wvd coloured by chain, front view.
PDB entry 8wvd coloured by chain, side view.
PDB entry 8wvd coloured by chain, top view.
Source organism: Siraitia grosvenorii
Primary publication:
Enzymatic Synthesis of Novel Terpenoid Glycoside Derivatives Decorated with N-Acetylglucosamine Catalyzed by UGT74AC1.
Li J, Li R, Shang N, Men Y, Cai Y, Zeng Y, Liu W, Yang J, Sun Y
J Agric Food Chem 72 14255-14263 (2024)
PMID: 38867497

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + mogrol = mogroside IE + UDP
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mogroside I-E synthase Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 51.22 KDa
Source organism: Siraitia grosvenorii
Expression system: Escherichia coli
UniProt:
  • Canonical: K7NBW3 (Residues: 1-454; Coverage: 100%)
Gene names: UDPG1, UGT74AC1
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase

Ligands and Environments

1 bound ligand:
Ligand UD1 1 x UD1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 49.867Å b: 68.825Å c: 142.689Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.233
Expression system: Escherichia coli

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