› 8wwh

Electron Microscopy
2.84Å resolution

MCHR1-Gi complex,S1 state

Released:
DOI: 10.2210/pdb8wwh/pdb
PDB entry 8wwh coloured by chain, front view.
PDB entry 8wwh coloured by chain, side view.
PDB entry 8wwh coloured by chain, top view.
Source organisms:
Entry authors: Gong W, Ye X, Liu G
Related structures: EMD-37888

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-281640 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.41 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 376 amino acids
Theoretical weight: 40.85 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Antibody fragment ScFv16 Chain: E
Molecule details ›
Chain: E
Length: 255 amino acids
Theoretical weight: 27.37 KDa
Source organism: synthetic construct
Expression system: Baculovirus expression vector pFastBac1-HM
Melanin-concentrating hormone receptor 1 Chain: R
Molecule details ›
Chain: R
Length: 624 amino acids
Theoretical weight: 68.21 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q99705 (Residues: 1-327; Coverage: 93%)
Gene names: GPR24, MCHR1, SLC1
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.84Å
Relevant EMDB volumes: EMD-37888
Expression system: Baculovirus expression vector pFastBac1-HM

Quick links