› 8wx0

Electron Microscopy
3.7Å resolution

PNPase of M.tuberculosis with its RNA substrate

Released:
DOI: 10.2210/pdb8wx0/pdb
PDB entry 8wx0 coloured by chain, front view.
PDB entry 8wx0 coloured by chain, side view.
PDB entry 8wx0 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures of Mycobacterium tuberculosis polynucleotide phosphorylase suggest a potential mechanism for its RNA substrate degradation.
Wang N, Sheng Y, Liu Y, Guo Y, He J, Liu J
Arch Biochem Biophys 754 109917 (2024)
PMID: 38395123
Related structures: EMD-37896

Function and Biology Details

Reaction catalysed: 
RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274253 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Polyribonucleotide nucleotidyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 773 amino acids
Theoretical weight: 82.12 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WI57 (Residues: 1-752; Coverage: 100%)
Gene names: Rv2783c, pnp
Sequence domains:
RNA (24-mer) Chain: E
Molecule details ›
Chain: E
Length: 24 nucleotides
Theoretical weight: 7.66 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (24-mer) Chains: D, F, G
Molecule details ›
Chains: D, F, G
Length: 24 nucleotides
Theoretical weight: 7.69 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-37896
Expression systems:
  • Escherichia coli
  • Not provided

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