8wxv

X-ray diffraction
2.4Å resolution

Structure of WDR5 in complex with WIN motif containing SET1B

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-246396 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
WD repeat-containing protein 5 Chains: A, C
Molecule details ›
Chains: A, C
Length: 315 amino acids
Theoretical weight: 34.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61964 (Residues: 24-334; Coverage: 93%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Histone-lysine N-methyltransferase SETD1B Chains: B, D
Molecule details ›
Chains: B, D
Length: 10 amino acids
Theoretical weight: 1.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UPS6 (Residues: 1745-1754; Coverage: 1%)
Gene names: KIAA1076, KMT2G, SET1B, SETD1B

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P1
Unit cell:
a: 46.904Å b: 61.499Å c: 64.774Å
α: 110.34° β: 91.27° γ: 112.39°
R-values:
R R work R free
0.171 0.168 0.205
Expression system: Escherichia coli