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Cryo-EM structure of DSR2-DSAD1 (partial) complex

Electron Microscopy
2.49Å resolution
PDB entry 8wye coloured by chain, front view.
PDB entry 8wye coloured by chain, side view.
PDB entry 8wye coloured by chain, top view.
Source organisms:
Assembly composition:
hetero pentamer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Bacillus phage SPbeta DSAD1 protein and SIR2-like domain-containing protein
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Multimeric state: hetero pentamer
Accessible surface area: 104134.64 Å2
Buried surface area: 18533.99 Å2
Dissociation area: 2,993.83 Å2
Dissociation energy (ΔGdiss): 0.8 kcal/mol
Dissociation entropy (TΔSdiss): 28.75 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-246243

Macromolecules

SIR2-like domain-containing protein
Chains: A, B, D, E
Length: 1005 amino acids
Theoretical weight: 118.64 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: D4G637 (Residues: 1-1005; Coverage: 100%)
Gene name: BSNT_07056
Pfam: SIR2-like domain
SIR2 family protein in PDB entry 8wye, assembly 1, front view.
SIR2 family protein in PDB entry 8wye, assembly 1, side view.
SIR2 family protein in PDB entry 8wye, assembly 1, top view.
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Bacillus phage SPbeta DSAD1 protein
Chain: C
Length: 146 amino acids
Theoretical weight: 16.59 KDa
Source organism: Bacillus phage SPBc2
Expression system: Escherichia coli
UniProt:
  • Canonical: O64191 (Residues: 1-120; Coverage: 100%)
Gene name: yotI
Bacillus phage SPbeta DSAD1 protein in PDB entry 8wye, assembly 1, front view.
Bacillus phage SPbeta DSAD1 protein in PDB entry 8wye, assembly 1, side view.
Bacillus phage SPbeta DSAD1 protein in PDB entry 8wye, assembly 1, top view.
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