› 8x0j

X-ray diffraction
3.11Å resolution

old yellow enzymes-Q102A/Y169F/R215A/R308A

Released:
DOI: 10.2210/pdb8x0j/pdb
PDB entry 8x0j coloured by chain, front view.
PDB entry 8x0j coloured by chain, side view.
PDB entry 8x0j coloured by chain, top view.
Source organism: Geobacillus kaustophilus HTA426
Entry authors: Lei W, Wei S

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 37.48 KDa
Source organism: Geobacillus kaustophilus HTA426
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5KXG9 (Residues: 1-340; Coverage: 100%)
Gene names: GK2332, namA
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family

Ligands and Environments

1 bound ligand:
Ligand PO4 9 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: I212121
Unit cell:
a: 86.06Å b: 143.57Å c: 155.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.265
Expression system: Escherichia coli

Quick links