Function and Biology

HIV-1 reverse transcriptase mutant Q151M/Y115F/F116Y/L74V:DNA:E-CFCP-TP ternary complex

Source organism: Human immunodeficiency virus 1
Biochemical function: not assigned
Biological process: not assigned
Cellular component: not assigned

EC 3.4.23.16: HIV-1 retropepsin

Reaction catalysed:
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Alternative Name(s):
  • Gag protease
  • HIV aspartyl protease
  • HIV proteinase
  • HIV-1 protease
  • HIV-2 protease
  • Human immunodeficiency virus type 1 protease
  • Retroproteinase

EC 3.1.26.13: Retroviral ribonuclease H

Reaction catalysed:
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Systematic name:
-
Alternative Name(s):
  • HIV RNase H
  • RT/RNase H
  • Retroviral reverse transcriptase RNaseH

EC 3.1.13.2: Exoribonuclease H

Reaction catalysed:
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Systematic name:
-

EC 2.7.7.7: DNA-directed DNA polymerase

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Systematic name:
Deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (DNA-directed)
Alternative Name(s):
  • DNA duplicase
  • DNA nucleotidyltransferase
  • DNA nucleotidyltransferase (DNA-directed)
  • DNA polymerase
  • DNA polymerase I
  • DNA polymerase II
  • DNA polymerase III
  • DNA polymerase alpha
  • DNA polymerase beta
  • DNA polymerase gamma
  • DNA replicase
  • DNA-dependent DNA polymerase
  • Deoxynucleate polymerase
  • Deoxyribonucleate nucleotidyltransferase
  • Deoxyribonucleic acid duplicase
  • Deoxyribonucleic acid polymerase
  • Deoxyribonucleic duplicase
  • Deoxyribonucleic polymerase
  • Deoxyribonucleic polymerase I
  • Duplicase
  • Klenow fragment
  • Sequenase
  • Taq DNA polymerase
  • Taq Pol I
  • Tca DNA polymerase

EC 2.7.7.49: RNA-directed DNA polymerase

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Systematic name:
Deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (RNA-directed)
Alternative Name(s):
  • DNA nucleotidyltransferase (RNA-directed)
  • RNA revertase
  • RNA-dependent DNA polymerase
  • RNA-dependent deoxyribonucleate nucleotidyltransferase
  • RNA-instructed DNA polymerase
  • RT
  • Reverse transcriptase
  • Revertase

GO terms

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Sequence families

Pfam Protein families (Pfam)
PF06815
Domain description: Reverse transcriptase connection domain
Occurring in:
  1. Reverse transcriptase/ribonuclease H
  2. p51 RT
The deposited structure of PDB entry 8x20 contains 2 copies of Pfam domain PF06815 (Reverse transcriptase connection domain) in Pol protein. Showing 1 copy in chain A.
The deposited structure of PDB entry 8x20 contains 2 copies of Pfam domain PF06815 (Reverse transcriptase connection domain) in p51 RT. Showing 1 copy in chain B.

PF06817
Domain description: Reverse transcriptase thumb domain
Occurring in:
  1. Reverse transcriptase/ribonuclease H
  2. p51 RT
The deposited structure of PDB entry 8x20 contains 2 copies of Pfam domain PF06817 (Reverse transcriptase thumb domain) in Pol protein. Showing 1 copy in chain A.
The deposited structure of PDB entry 8x20 contains 2 copies of Pfam domain PF06817 (Reverse transcriptase thumb domain) in p51 RT. Showing 1 copy in chain B.

PF00078
Domain description: Reverse transcriptase (RNA-dependent DNA polymerase)
Occurring in:
  1. Reverse transcriptase/ribonuclease H
  2. p51 RT
The deposited structure of PDB entry 8x20 contains 2 copies of Pfam domain PF00078 (Reverse transcriptase (RNA-dependent DNA polymerase)) in Pol protein. Showing 1 copy in chain A.
The deposited structure of PDB entry 8x20 contains 2 copies of Pfam domain PF00078 (Reverse transcriptase (RNA-dependent DNA polymerase)) in p51 RT. Showing 1 copy in chain B.

PF00075
Domain description: RNase H
Occurring in:
  1. Reverse transcriptase/ribonuclease H
The deposited structure of PDB entry 8x20 contains 2 copies of Pfam domain PF00075 (RNase H) in Pol protein. Showing 1 copy in chain A.