8x2v

X-ray diffraction
1.29Å resolution

Crystal structure of the ancestral GH19 chitinase, Anc4+LoopII (P12K/N13H mutant)

Released:
Source organism: synthetic construct
Entry authors: Kozome D, Laurino P

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-242662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH19 chitinase Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.46 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Unit cell:
a: 37.29Å b: 69.83Å c: 70.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.186
Expression system: Escherichia coli BL21(DE3)