› 8x2x

Electron Microscopy
3.8Å resolution

The piccolo NuA4 bound to the H2A.Z nucleosome complex at pre-H4-acetylation state

Released:
DOI: 10.2210/pdb8x2x/pdb
PDB entry 8x2x coloured by chain, front view.
PDB entry 8x2x coloured by chain, side view.
PDB entry 8x2x coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures reveal the acetylation process of piccolo NuA4.
Wang L, Zhang H, Jia Q, Li W, Yang C, Ma L, Li M, Lu Y, Zhu H, Zhu P
Proc Natl Acad Sci U S A 122 e2414490122 (2025)
PMID: 40100634
Related structures: EMD-38021

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-290491 (preferred)
Entry contents:
8 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (9 distinct):
Maltose/maltodextrin-binding periplasmic protein Chain: L
Molecule details ›
Chain: L
Length: 537 amino acids
Theoretical weight: 59.47 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0AEX9 (Residues: 1-392; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Chromatin modification-related protein Chain: N
Molecule details ›
Chain: N
Length: 120 amino acids
Theoretical weight: 13.83 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Histone acetyltransferase Chain: K
glutathione transferase Chain: M
Histone H3 Chains: A, E
Histone H4 Chains: B, F
Histone H2A Chains: C, G
Histone H2B Chains: D, H
DNA (146-MER) Chains: I, J

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.8Å
Relevant EMDB volumes: EMD-38021
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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