› 8x3s

X-ray diffraction
1.87Å resolution

Crystal structure of human WDR5 in complex with PTEN

Released:
DOI: 10.2210/pdb8x3s/pdb
PDB entry 8x3s coloured by chain, front view.
PDB entry 8x3s coloured by chain, side view.
PDB entry 8x3s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The NTE domain of PTENα/β promotes cancer progression by interacting with WDR5 via its SSSRRSS motif.
Huang X, Zhang C, Shang X, Chen Y, Xiao Q, Wei Z, Wang G, Zhen X, Xu G, Min J, Shen S, Liu Y
OpenAccess logo Cell Death Dis 15 335 (2024)
PMID: 38744853

Function and Biology Details

Reactions catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Phosphatidylinositol 3,4,5-trisphosphate + H(2)O = phosphatidylinositol 4,5-bisphosphate + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244820 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 34.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61964 (Residues: 21-334; Coverage: 94%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Chain: B
Molecule details ›
Chain: B
Length: 34 amino acids
Theoretical weight: 3.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60484 (Residues: 29-41; Coverage: 3%)
  • Best match: P60484-2 (Residues: 115-148)
Gene names: MMAC1, PTEN, TEP1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL10U2
Spacegroup: P212121
Unit cell:
a: 46.46Å b: 63.75Å c: 92.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.259
Expression system: Escherichia coli

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