› 8x44

X-ray diffraction
2.6Å resolution

Crystal structure of DIMT1 in complex with 5'-methylthioadenosine from Pyrococcus horikoshii (FormI)

Released:
DOI: 10.2210/pdb8x44/pdb
PDB entry 8x44 coloured by chain, front view.
PDB entry 8x44 coloured by chain, side view.
PDB entry 8x44 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Structural and functional characterization of archaeal DIMT1 unveils distinct protein dynamics essential for efficient catalysis.
Saha S, Kanaujia SP
Structure (2024)
PMID: 39146930

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-250753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable ribosomal RNA small subunit methyltransferase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 33.37 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

7 bound ligands:
Ligand MTA 2 x MTA
Ligand ZN 8 x ZN
Ligand SO3 4 x SO3
Ligand PEG 4 x PEG
Ligand CL 1 x CL
Ligand GOL 1 x GOL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Unit cell:
a: 79.78Å b: 85.34Å c: 106.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.273
Expression system: Escherichia coli BL21(DE3)

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