› 8x5d

Electron Microscopy
3.1Å resolution

The cryo-EM structure of the Mycobacterium tuberculosis CRISPR-Csm complex

Released:
DOI: 10.2210/pdb8x5d/pdb
PDB entry 8x5d coloured by chain, front view.
PDB entry 8x5d coloured by chain, side view.
PDB entry 8x5d coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Type-III-A structure of mycobacteria CRISPR-Csm complexes involving atypical crRNAs.
Zhang H, Shi M, Ma X, Liu M, Wang N, Lu Q, Li Z, Zhao Y, Zhao H, Chen H, Zhang H, Jiang T, Ouyang S, Huo Y, Bi L
Int J Biol Macromol 260 129331 (2024)
PMID: 38218299
Related structures: EMD-38066

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-245801 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR system Cms endoribonuclease Csm3 Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 239 amino acids
Theoretical weight: 26.15 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WJF9 (Residues: 1-236; Coverage: 100%)
Gene names: Rv2821c, csm3
Sequence domains: RAMP superfamily
CRISPR system Cms protein Csm5 Chain: N
Molecule details ›
Chain: N
Length: 378 amino acids
Theoretical weight: 42.75 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WJF5 (Residues: 1-375; Coverage: 100%)
Gene names: Rv2819c, csm5
Sequence domains: RAMP superfamily
Csm2 Chains: B, C, D, E, F
Molecule details ›
Chains: B, C, D, E, F
Length: 133 amino acids
Theoretical weight: 15.35 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
RNA (47-MER) Chain: O
Molecule details ›
Chain: O
Length: 184 nucleotides
Theoretical weight: 59.36 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-38066
Expression system: Escherichia coli

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