› 8xe5

X-ray diffraction
2.07Å resolution

norbelladine 4'-O-methyltransferase complexed with Mg, SAH, and 3,4-dihydroxybenzaldehyde

Released:
DOI: 10.2210/pdb8xe5/pdb
PDB entry 8xe5 coloured by chain, front view.
PDB entry 8xe5 coloured by chain, side view.
PDB entry 8xe5 coloured by chain, top view.
Source organism: Narcissus aff. pseudonarcissus MK-2014
Primary publication:
Structure-Based Catalytic Mechanism of Amaryllidaceae O-Methyltransferases
Hnin SYY, Nakashima Y, Kodama T, Morita H
ACS Catalysis 11865-11880 (2024)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + norbelladine = S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Norbelladine 4'-O-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 242 amino acids
Theoretical weight: 27.4 KDa
Source organism: Narcissus aff. pseudonarcissus MK-2014
Expression system: Escherichia coli M15
UniProt:
  • Canonical: A0A077EWA5 (Residues: 1-239; Coverage: 100%)
Gene name: N4OMT
Sequence domains: O-methyltransferase

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand SAH 4 x SAH
Ligand H6N 2 x H6N
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P21
Unit cell:
a: 64.328Å b: 79.901Å c: 91.286Å
α: 90° β: 93.862° γ: 90°
R-values:
R R work R free
0.193 0.191 0.225
Expression system: Escherichia coli M15

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