› 8xh1

X-ray diffraction
1.7Å resolution

Orthorhombic crystal structure of green fluorescent protein nowGFP at pH 9.0

Released:
DOI: 10.2210/pdb8xh1/pdb
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PDB entry 8xh1 coloured by chain, front view.
PDB entry 8xh1 coloured by chain, side view.
PDB entry 8xh1 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Comparison of two crystal polymorphs of NowGFP reveals a new conformational state trapped by crystal packing.
Kim JK, Jeong H, Seo J, Kim S, Kim KH, Min D, Kim CU
Acta Crystallogr D Struct Biol (2024)
PMID: 39222305

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-251360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
nowGFP Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 26.66 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand CRF 2 x CRF

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 51.244Å b: 52.28Å c: 195.295Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.153 0.22
Expression system: Escherichia coli

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