› 8xha

X-ray diffraction
2.27Å resolution

Crystal structure of alpha-Oxoamine Synthase Alb29 with PLP cofactor and L-glutamate

Released:
DOI: 10.2210/pdb8xha/pdb
PDB entry 8xha coloured by chain, front view.
PDB entry 8xha coloured by chain, side view.
PDB entry 8xha coloured by chain, top view.
Source organism: Streptomyces albogriseolus 1-36
Primary publication:
Structural and mechanistic investigations on CC bond forming α-oxoamine synthase allowing L-glutamate as substrate.
Zhang DK, Song KY, Yan YQ, Zheng JT, Xu J, Da LT, Xu MJ
Int J Biol Macromol 131696 (2024)
PMID: 38642679

Function and Biology Details

Reaction catalysed: 
Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO(2) + holo-[acyl-carrier protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
8-amino-7-oxononanoate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 43.33 KDa
Source organism: Streptomyces albogriseolus 1-36
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A6B9KSL0 (Residues: 11-405; Coverage: 97%)
Gene name: alb29
Sequence domains: Aminotransferase class I and II

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand PGU 1 x PGU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P3221
Unit cell:
a: 117.369Å b: 117.369Å c: 145.106Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.224
Expression system: Escherichia coli BL21(DE3)

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