› 8xhr

X-ray diffraction
2.1Å resolution

Crystal structure of Mycobacterium tuberculosis MenT3 bound with CTP

Released:
DOI: 10.2210/pdb8xhr/pdb
PDB entry 8xhr coloured by chain, front view.
PDB entry 8xhr coloured by chain, side view.
PDB entry 8xhr coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Substrate specificity of Mycobacterium tuberculosis tRNA terminal nucleotidyltransferase toxin MenT3.
Liu J, Yashiro Y, Sakaguchi Y, Suzuki T, Tomita K
Nucleic Acids Res (2024)
PMID: 38485701

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161216 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleotidyl transferase AbiEii/AbiGii toxin family protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 301 amino acids
Theoretical weight: 33.06 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P96356 (Residues: 1-293; Coverage: 100%)
Gene name: Rv1045
Sequence domains: Nucleotidyl transferase AbiEii toxin, Type IV TA system

Ligands and Environments

1 bound ligand:
Ligand CTP 3 x CTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P21
Unit cell:
a: 59.96Å b: 91.53Å c: 85.06Å
α: 90° β: 101° γ: 90°
R-values:
R R work R free
0.19 0.188 0.241
Expression system: Escherichia coli BL21(DE3)

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