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Electron Microscopy
2.84Å resolution

Structure of the Argonaute protein from Kurthia massiliensis in complex with guide DNA and 19-mer target RNA

Released:
Source organism: Kurthia massiliensis
Primary publication:
Structural and mechanistic insights into a mesophilic prokaryotic Argonaute.
Nucleic Acids Res 52 11895-11910 (2024)
PMID: 39315697
Related structures: EMD-38408

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-282721 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
KmAgo Chain: A
Molecule details ›
Chain: A
Length: 737 amino acids
Theoretical weight: 85.49 KDa
Source organism: Kurthia massiliensis
Expression system: Escherichia coli
guide DNA Chain: B
Molecule details ›
Chain: B
Length: 18 nucleotides
Theoretical weight: 5.64 KDa
Source organism: Kurthia massiliensis
Expression system: Not provided
target RNA Chain: C

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.84Å
Relevant EMDB volumes: EMD-38408
Expression systems:
  • Escherichia coli
  • Not provided