8xk9

X-ray diffraction
2.4Å resolution

ternary complex of DNA polymerase SFM4-3 recognizing C2 methyoxy nucleotide

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-281315 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase I, thermostable Chains: A, D
Molecule details ›
Chains: A, D
Length: 539 amino acids
Theoretical weight: 60.8 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19821 (Residues: 294-832; Coverage: 65%)
Gene names: pol1, polA
Sequence domains:
DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 12 nucleotides
Theoretical weight: 3.62 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*AP*CP*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-R(P*(OMG))-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 16 nucleotides
Theoretical weight: 4.97 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P43
Unit cell:
a: 105.986Å b: 105.986Å c: 191.722Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.218
Expression systems:
  • Escherichia coli
  • Not provided