8xm1

X-ray diffraction
1.9Å resolution

Phytase mutant APPAmut4

Released:
Source organism: Yersinia intermedia
Entry authors: Tu T, Wang Q

Function and Biology Details

Reaction catalysed:
Myo-inositol hexakisphosphate + H(2)O = 1D-myo-inositol 1,2,3,5,6-pentakisphosphate + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytase Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 45.62 KDa
Source organism: Yersinia intermedia
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q000T0 (Residues: 24-441; Coverage: 100%)
Sequence domains: Histidine phosphatase superfamily (branch 2)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL10U2
Spacegroup: P212121
Unit cell:
a: 43.514Å b: 46.016Å c: 192.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.158 0.208
Expression system: Komagataella pastoris