› 8xm7

Electron Microscopy
4.91Å resolution

Cryo-EM structure of the RhoG/DOCK5/ELMO1/Rac1 complex: RhoG/DOCK5/ELMO1 focused map

Released:
DOI: 10.2210/pdb8xm7/pdb
PDB entry 8xm7 coloured by chain, front view.
PDB entry 8xm7 coloured by chain, side view.
PDB entry 8xm7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
RhoG facilitates a conformational transition in the guanine nucleotide exchange factor complex DOCK5/ELMO1 to an open state.
Kukimoto-Niino M, Katsura K, Ishizuka-Katsura Y, Mishima-Tsumagari C, Yonemochi M, Inoue M, Nakagawa R, Kaushik R, Zhang KYJ, Shirouzu M
J Biol Chem 107459 (2024)
PMID: 38857861
Related structures: EMD-38466

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-274206 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Engulfment and cell motility protein 1 Chain: A
Molecule details ›
Chain: A
Length: 733 amino acids
Theoretical weight: 84.34 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q92556 (Residues: 1-727; Coverage: 100%)
Gene names: ELMO1, KIAA0281
Sequence domains:
Dedicator of cytokinesis protein 5 Chain: B
Molecule details ›
Chain: B
Length: 1648 amino acids
Theoretical weight: 191.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9H7D0 (Residues: 1-1642; Coverage: 88%)
Gene name: DOCK5
Sequence domains:
Rho-related GTP-binding protein RhoG Chain: D
Molecule details ›
Chain: D
Length: 203 amino acids
Theoretical weight: 22.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P84095 (Residues: 1-184; Coverage: 96%)
Gene names: ARHG, RHOG
Sequence domains: Ras family

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.91Å
Relevant EMDB volumes: EMD-38466
Expression systems:
  • Homo sapiens
  • Escherichia coli

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