› 8xoj

Electron Microscopy
3.1Å resolution

Cryo-EM structure of GPR30-Gq complex structure in the presence of G-1

Released:
DOI: 10.2210/pdb8xoj/pdb
PDB entry 8xoj coloured by chain, front view.
PDB entry 8xoj coloured by chain, side view.
PDB entry 8xoj coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional evidence that GPR30 is not a direct estrogen receptor.
Liu H, Guo S, Dai A, Xu P, Li X, Huang S, He X, Wu K, Zhang X, Yang D, Xie X, Xu HE
Cell Res (2024)
PMID: 38744981
Related structures: EMD-38531

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-548136 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.63 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-19, 32-32, 61-181, 229-229, 251-253, 309-309, 319-319, 347-348; Coverage: 42%)
  • Canonical: P63092 (Residues: 27-38, 40-67, 205-251, 263-273, 277-346, 348-357, 359-386, 389-394; Coverage: 54%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 247 amino acids
Theoretical weight: 26.29 KDa
Source organism: synthetic construct
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
G-protein coupled estrogen receptor 1 Chain: R
Molecule details ›
Chain: R
Length: 375 amino acids
Theoretical weight: 42.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q99527 (Residues: 1-375; Coverage: 100%)
Gene names: CEPR, CMKRL2, DRY12, GPER, GPER1, GPR30
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-38531
Expression system: Spodoptera frugiperda

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