8xu8

Electron Microscopy
3.4Å resolution

State 2c(S2c) of yeast 80S ribosome bound to compact eEF2 and 2 tRNAs during peptidyl transferation

Released:
Entry authors: Cheng J, Wu CL, Li JX, Zhang XZ
Related structures: EMD-38660

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 81-mer (preferred)
PDBe Complex ID:
PDB-CPX-282106 (preferred)
Entry contents:
75 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (80 distinct):
Large ribosomal subunit protein uL2A Chain: D
Molecule details ›
Chain: D
Length: 251 amino acids
Theoretical weight: 27.09 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX45 (Residues: 2-252; Coverage: 99%)
Gene names: RPL2A, RPL5B, YFR031BC, YFR031C-A
Sequence domains:
Large ribosomal subunit protein uL3 Chain: E
Molecule details ›
Chain: E
Length: 386 amino acids
Theoretical weight: 43.72 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P14126 (Residues: 2-387; Coverage: 100%)
Gene names: MAK8, RPL3, TCM1, YOR063W, YOR29-14
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4A Chain: F
Large ribosomal subunit protein uL18 Chain: G
Large ribosomal subunit protein eL6B Chain: H
Large ribosomal subunit protein uL30A Chain: I
Large ribosomal subunit protein eL8A Chain: J
Large ribosomal subunit protein uL6A Chain: K
Large ribosomal subunit protein uL16 Chain: L
Large ribosomal subunit protein uL5B Chain: M
Large ribosomal subunit protein eL13A Chain: N
Large ribosomal subunit protein eL14A Chain: O
Large ribosomal subunit protein eL15A Chain: P
Large ribosomal subunit protein uL13A Chain: Q
Large ribosomal subunit protein uL22A Chain: R
Large ribosomal subunit protein eL18A Chain: S
Large ribosomal subunit protein eL19A Chain: T
Large ribosomal subunit protein eL20A Chain: U
Large ribosomal subunit protein eL21A Chain: V
Large ribosomal subunit protein eL22A Chain: W
Large ribosomal subunit protein uL14A Chain: X
Large ribosomal subunit protein eL24A Chain: Y
Large ribosomal subunit protein uL23 Chain: Z
Large ribosomal subunit protein uL24A Chain: a
Large ribosomal subunit protein eL27A Chain: b
Large ribosomal subunit protein uL15 Chain: c
Large ribosomal subunit protein eL29 Chain: d
Large ribosomal subunit protein eL30 Chain: e
Large ribosomal subunit protein eL31A Chain: f
Large ribosomal subunit protein eL32 Chain: g
Large ribosomal subunit protein eL33A Chain: h
Large ribosomal subunit protein eL34A Chain: i
Large ribosomal subunit protein uL29A Chain: j
Large ribosomal subunit protein eL36A Chain: k
Large ribosomal subunit protein eL37A Chain: l
Large ribosomal subunit protein eL38 Chain: m
Large ribosomal subunit protein eL39 Chain: n
Large ribosomal subunit protein eL40A Chain: o
Small ribosomal subunit protein eS32A Chain: p
Large ribosomal subunit protein eL42A Chain: q
Large ribosomal subunit protein eL43A Chain: r
Elongation factor 2 Chain: x
Small ribosomal subunit protein eS12 Chain: SD
Small ribosomal subunit protein uS11B Chain: SZ
Small ribosomal subunit protein eS26A Chain: Se
Small ribosomal subunit protein eS10A Chain: SC
Small ribosomal subunit protein uS19 Chain: SE
Small ribosomal subunit protein uS3 Chain: SA
Small ribosomal subunit protein eS19A Chain: SI
Small ribosomal subunit protein uS10 Chain: SJ
Small ribosomal subunit protein eS28A Chain: SL
Small ribosomal subunit protein uS14A Chain: SM
Small ribosomal subunit protein uS7 Chain: SB
Small ribosomal subunit protein uS9A Chain: SF
Small ribosomal subunit protein uS13A Chain: SH
Small ribosomal subunit protein RACK1 Chain: SO
Small ribosomal subunit protein eS31 Chain: SN
Small ribosomal subunit protein eS17A Chain: SG
Small ribosomal subunit protein uS2A Chain: SP
Small ribosomal subunit protein eS1A Chain: SQ
Small ribosomal subunit protein uS5 Chain: SR
Small ribosomal subunit protein eS4A Chain: SS
Small ribosomal subunit protein eS6A Chain: ST
Small ribosomal subunit protein eS7A Chain: SU
Small ribosomal subunit protein eS8A Chain: SV
Small ribosomal subunit protein uS4A Chain: SW
Small ribosomal subunit protein uS17A Chain: SX
Small ribosomal subunit protein uS15 Chain: SY
Small ribosomal subunit protein eS21A Chain: Sa
Small ribosomal subunit protein uS8A Chain: Sb
Small ribosomal subunit protein uS12A Chain: Sc
Small ribosomal subunit protein eS24A Chain: Sd
Small ribosomal subunit protein eS27A Chain: Sf
Small ribosomal subunit protein eS30A Chain: Sg
Small ribosomal subunit protein eS25A Chain: SK
18S Ribosomal RNA Chain: 2
25S ribosomal RNA Chain: A
5S ribosomal RNA Chain: B
5.8S ribosomal RNA Chain: C
tRNA Chains: s, t

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-38660