› 8xwq

Electron Microscopy
4.6Å resolution

Cryo-EM structure of ET-1 bound ETBR-DNGI complex

Released:
DOI: 10.2210/pdb8xwq/pdb
PDB entry 8xwq coloured by chain, front view.
PDB entry 8xwq coloured by chain, side view.
PDB entry 8xwq coloured by chain, top view.
Source organisms:
Primary publication:
Structure of endothelin ETB receptor-Gi complex in a conformation stabilized by unique NPxxL motif.
Tani K, Maki-Yonekura S, Kanno R, Negami T, Hamaguchi T, Hall M, Mizoguchi A, Humbel BM, Terada T, Yonekura K, Doi T
Commun Biol 7 1303 (2024)
PMID: 39414992
Related structures: EMD-38741

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-272325 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Endothelin receptor type B Chain: R
Molecule details ›
Chain: R
Length: 346 amino acids
Theoretical weight: 38.81 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P24530 (Residues: 66-407; Coverage: 82%)
Gene names: EDNRB, ETRB
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Endothelin-1 Chain: L
Molecule details ›
Chain: L
Length: 21 amino acids
Theoretical weight: 2.5 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P05305 (Residues: 53-73; Coverage: 11%)
Gene name: EDN1
Sequence domains: Endothelin family
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 344 amino acids
Theoretical weight: 37.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
SCFV16 Chain: D
Molecule details ›
Chain: D
Length: 277 amino acids
Theoretical weight: 29.4 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-38741
Expression system: Spodoptera frugiperda

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