› 8xzi

Electron Microscopy
2.7Å resolution

Cryo-EM structure of the CMF-019-bound human APLNR-Gi complex

Released:
DOI: 10.2210/pdb8xzi/pdb
PDB entry 8xzi coloured by chain, front view.
PDB entry 8xzi coloured by chain, side view.
PDB entry 8xzi coloured by chain, top view.
Source organisms:
Primary publication:
Structure-based design of non-hypertrophic apelin receptor modulator.
Wang WW, Ji SY, Zhang W, Zhang J, Cai C, Hu R, Zang SK, Miao L, Xu H, Chen LN, Yang Z, Guo J, Qin J, Shen DD, Liang P, Zhang Y, Zhang Y
Cell 187 1460-1475.e20 (2024)
PMID: 38428423
Related structures: EMD-38797

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-241935 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Apelin receptor Chain: R
Molecule details ›
Chain: R
Length: 380 amino acids
Theoretical weight: 42.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P35414 (Residues: 1-380; Coverage: 100%)
Gene names: AGTRL1, APJ, APLNR
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.41 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 250 amino acids
Theoretical weight: 26.61 KDa
Source organism: synthetic construct
Expression system: Trichoplusia ni

Ligands and Environments

1 bound ligand:
Ligand A1D5N 1 x A1D5N
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-38797
Expression systems:
  • Spodoptera frugiperda
  • Trichoplusia ni

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