8y1k

Electron Microscopy
3.1Å resolution

The cryo-EM structure of TdpAB in complex with AMPPNP and PT-DNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-284499 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
TdpA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 586 amino acids
Theoretical weight: 66.55 KDa
Source organism: Thermus antranikianii DSM 12462
Expression system: Escherichia coli BL21(DE3)
TdpB Chains: G, H
Molecule details ›
Chains: G, H
Length: 375 amino acids
Theoretical weight: 42.76 KDa
Source organism: Thermus antranikianii DSM 12462
Expression system: Escherichia coli BL21(DE3)
DNA (5'-D(P*GP*CP*CP*CP*TP*TP*TP*TP*GP*CP*AP*A)-3') Chain: I
DNA (5'-D(P*TP*TP*GP*CP*AP*AP*AP*AP*GP*GP*GP*C)-3') Chain: J

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-38837
Expression system: Escherichia coli BL21(DE3)