› 8y27

X-ray diffraction
1.45Å resolution

X-ray crystal structure of ALiS5-Streptavidine complex with 10% glycerol using a high-pressure cryocooling method

Released:
DOI: 10.2210/pdb8y27/pdb
Model geometry
Fit model/data
PDB entry 8y27 coloured by chain, front view.
PDB entry 8y27 coloured by chain, side view.
PDB entry 8y27 coloured by chain, top view.
Source organism: Streptomyces avidinii
Entry authors: Higashiura A, Yamamoto A, Sugiyama S

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 121 amino acids
Theoretical weight: 12.76 KDa
Source organism: Streptomyces avidinii
UniProt:
  • Canonical: P22629 (Residues: 39-159; Coverage: 76%)
Sequence domains: Avidin family

Ligands and Environments

3 bound ligands:
Ligand DMS 1 x DMS
Ligand GOL 3 x GOL
Ligand A1LXQ 3 x A1LXQ
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 46.988Å b: 85.539Å c: 58.367Å
α: 90° β: 98.92° γ: 90°
R-values:
R R work R free
0.142 0.139 0.186

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