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X-ray crystal structure of ALiS2-Streptavidine complex with 10% glycerol using a high-pressure cryocooling method

X-ray diffraction
1.34Å resolution
PDB entry 8y2a coloured by chain, front view.
PDB entry 8y2a coloured by chain, side view.
PDB entry 8y2a coloured by chain, top view.
Source organism: Streptomyces avidinii
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Streptavidin
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Multimeric state: homo tetramer
Accessible surface area: 19297.92 Å2
Buried surface area: 9025.76 Å2
Dissociation area: 1,403.8 Å2
Dissociation energy (ΔGdiss): 12.9 kcal/mol
Dissociation entropy (TΔSdiss): 12.66 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-149694

Macromolecules

Streptavidin
Chains: A, B, C, D
Length: 121 amino acids
Theoretical weight: 12.76 KDa
Source organism: Streptomyces avidinii
UniProt:
  • Canonical: P22629 (Residues: 39-159; Coverage: 76%)
Pfam: Avidin family
Streptavidin in PDB entry 8y2a, assembly 1, front view.
Streptavidin in PDB entry 8y2a, assembly 1, side view.
Streptavidin in PDB entry 8y2a, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P22629  
1121102030405060708090100110120
 
50100
UniProt
P22629
Chains
Ligand binding sites
Interaction interfaces
Molecule details ›

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