› 8y2h

Electron Microscopy
3.3Å resolution

GK tetramer of AtP5CS1 filament with adjacent hooks, reaction state

Released:
DOI: 10.2210/pdb8y2h/pdb
PDB entry 8y2h coloured by chain, front view.
PDB entry 8y2h coloured by chain, side view.
PDB entry 8y2h coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Dynamic Arabidopsis P5CS filament facilitates substrate channelling.
Guo CJ, Zhang T, Leng Q, Zhou X, Zhong J, Liu JL
Nat Plants (2024)
PMID: 38740943
Related structures: EMD-38855

Function and Biology Details

Reactions catalysed: 
L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH
ATP + L-glutamate = ADP + L-glutamate 5-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245101 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-1-pyrroline-5-carboxylate synthase A Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 727 amino acids
Theoretical weight: 78.99 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54887 (Residues: 1-717; Coverage: 100%)
Gene names: At2g39800, P5CS1, P5CSA, T5I7.10
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ATP 4 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-38855
Expression system: Escherichia coli BL21(DE3)

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