Function and Biology Details
Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
A 5'-triphospho-[mRNA] + H(2)O = a 5'-diphospho-[mRNA] + phosphate
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
mRNA-capping enzyme (preferred)
PDBe Complex ID:
PDB-CPX-284442 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
mRNA-capping enzyme catalytic subunit
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 34.63 KDa
Source organism: Monkeypox virus
Expression system: Escherichia
UniProt:
Sequence domains: mRNA (guanine-N(7))-methyltransferase domain
Length: 300 amino acids
Theoretical weight: 34.63 KDa
Source organism: Monkeypox virus
Expression system: Escherichia
UniProt:
- Canonical:
A0A7H0DN85 (Residues: 546-845; Coverage: 36%)
Sequence domains: mRNA (guanine-N(7))-methyltransferase domain
mRNA-capping enzyme regulatory subunit OPG124
Molecule details ›
Chain: B
Length: 287 amino acids
Theoretical weight: 33.5 KDa
Source organism: Monkeypox virus
Expression system: Escherichia coli
UniProt:
Sequence domains: Poxvirus mRNA capping enzyme, small subunit
Length: 287 amino acids
Theoretical weight: 33.5 KDa
Source organism: Monkeypox virus
Expression system: Escherichia coli
UniProt:
- Canonical: A0A7H0DN96 (Residues: 1-287; Coverage: 100%)
Sequence domains: Poxvirus mRNA capping enzyme, small subunit
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this entry.
X-ray source:
SSRF BEAMLINE BL10U2
Spacegroup:
P6522
Expression systems:
- Escherichia
- Escherichia coli
