8y3h

Electron Microscopy
2.8Å resolution

Cryo-EM structure of dengue virus serotype 2 strain D2Y98P-PP1 N153Q mutant in complex with human antibody 2D22 Fab at 37 deg C

Released:
Entry authors: Fibriansah G, Ng TS, Lim XN, Tan AWK, Shi J, Lok SM
Related structures: EMD-38882

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 720-mer (preferred)
PDBe Complex ID:
PDB-CPX-284438 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Envelope protein Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 495 amino acids
Theoretical weight: 54.3 KDa
Source organism: dengue virus type 2
Expression system: Aedes albopictus
UniProt:
  • Canonical: A0A678XB23 (Residues: 1-495; Coverage: 100%)
Gene name: E
Sequence domains:
Membrane polyprotein Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 75 amino acids
Theoretical weight: 8.38 KDa
Source organism: dengue virus type 2
Expression system: Aedes albopictus
UniProt:
  • Canonical: A4GVG9 (Residues: 206-280; Coverage: 9%)
Sequence domains: Flavivirus envelope glycoprotein M
Human antibody 2D22 Fab heavy chain Chains: G, I, K
Human antibody 2D22 Fab light chain Chains: H, J, L

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-38882
Expression systems:
  • Aedes albopictus
  • Homo sapiens