› 8y89

Electron Microscopy
3.32Å resolution

Structure of HCoV-HKU1C spike in the functionally anchored-3up conformation with 2TMPRSS2

Released:
DOI: 10.2210/pdb8y89/pdb
PDB entry 8y89 coloured by chain, front view.
PDB entry 8y89 coloured by chain, side view.
PDB entry 8y89 coloured by chain, top view.
Source organisms:
Primary publication:
TMPRSS2 and glycan receptors synergistically facilitate coronavirus entry.
Wang H, Liu X, Zhang X, Zhao Z, Lu Y, Pu D, Zhang Z, Chen J, Wang Y, Li M, Dong X, Duan Y, He Y, Mao Q, Guo H, Sun H, Zhou Y, Yang Q, Gao Y, Yang X, Cao H, Guddat L, Sun L, Rao Z, Yang H
Cell (2024)
PMID: 38964329
Related structures: EMD-39038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-247132 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Spike glycoprotein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1263 amino acids
Theoretical weight: 140.24 KDa
Source organism: Human coronavirus HKU1 (isolate N5)
Expression system: Homo sapiens
UniProt:
  • Canonical: Q0ZME7 (Residues: 14-1276; Coverage: 94%)
Gene names: 3, S
Sequence domains:
Transmembrane protease serine 2 non-catalytic chain Chains: G, T
Molecule details ›
Chains: G, T
Length: 383 amino acids
Theoretical weight: 42.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O15393 (Residues: 109-492; Coverage: 78%)
Gene names: PRSS10, TMPRSS2
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 13 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.32Å
Relevant EMDB volumes: EMD-39038
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

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