› 8yaa

Electron Microscopy
3.34Å resolution

Cryo-EM structure of MIK2-SCOOP12-BAK1

Released:
DOI: 10.2210/pdb8yaa/pdb
PDB entry 8yaa coloured by chain, front view.
PDB entry 8yaa coloured by chain, side view.
PDB entry 8yaa coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
N-glycosylation facilitates the activation of a plant cell-surface receptor.
Jia F, Xiao Y, Feng Y, Yan J, Fan M, Sun Y, Huang S, Li W, Zhao T, Han Z, Hou S, Chai J
Nat Plants (2024)
PMID: 39511417
Related structures: EMD-39093

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-280455 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (6 distinct):
BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 Chain: C
Molecule details ›
Chain: C
Length: 177 amino acids
Theoretical weight: 19.16 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q94F62 (Residues: 26-202; Coverage: 30%)
Gene names: At4g33430, BAK1, ELG, F17M5.190, SERK3
Sequence domains:
MDIS1-interacting receptor like kinase 2 Chain: A
Molecule details ›
Chain: A
Length: 654 amino acids
Theoretical weight: 71.86 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8VZG8 (Residues: 43-696; Coverage: 65%)
Gene names: At4g08850, MIK2, T32A17.160
Sequence domains:
Serine rich endogenous peptide 12 Chain: B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 8 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.34Å
Relevant EMDB volumes: EMD-39093
Expression systems:
  • Trichoplusia ni
  • Not provided

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