› 8ybx

Electron Microscopy
3.68Å resolution

Structure of the FADD/Caspase-8/cFLIP death effector domain assembly

Released:
DOI: 10.2210/pdb8ybx/pdb
PDB entry 8ybx coloured by chain, front view.
PDB entry 8ybx coloured by chain, side view.
PDB entry 8ybx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Deciphering DED assembly mechanisms in FADD-procaspase-8-cFLIP complexes regulating apoptosis.
Yang CY, Lien CI, Tseng YC, Tu YF, Kulczyk AW, Lu YC, Wang YT, Su TW, Hsu LC, Lo YC, Lin SC
OpenAccess logo Nat Commun 15 3791 (2024)
PMID: 38710704
Related structures: EMD-39126

Function and Biology Details

Reaction catalysed: 
Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-244371 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Caspase-8 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 479 amino acids
Theoretical weight: 55.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q14790 (Residues: 1-479; Coverage: 100%)
Gene names: CASP8, MCH5
Sequence domains:
CASP8 and FADD-like apoptosis regulator subunit p43 Chains: H, I, J, K
Molecule details ›
Chains: H, I, J, K
Length: 181 amino acids
Theoretical weight: 20.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O15519 (Residues: 1-181; Coverage: 38%)
Gene names: CASH, CASP8AP1, CFLAR, CLARP, MRIT
Sequence domains: Death effector domain
FAS-associated death domain protein Chains: L, Q, R
Molecule details ›
Chains: L, Q, R
Length: 216 amino acids
Theoretical weight: 24.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q13158 (Residues: 1-208; Coverage: 100%)
Gene names: FADD, GIG3, MORT1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.68Å
Relevant EMDB volumes: EMD-39126
Expression system: Escherichia coli BL21

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