8ye9

Electron Microscopy
3.43Å resolution

Cryo-EM structure of Cas9-sgRNA-A25 complex

Released:
Primary publication:
Inhibition mechanisms of CRISPR-Cas9 by AcrIIA25.1 and AcrIIA32.
Sci China Life Sci 67 1781-1791 (2024)
PMID: 38842649
Related structures: EMD-39191

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-274393 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1368 amino acids
Theoretical weight: 158.7 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
A25 Chain: B
Molecule details ›
Chain: B
Length: 101 amino acids
Theoretical weight: 11.9 KDa
Source organism: Streptococcus sp. F0441
Expression system: Escherichia coli
RNA (98-MER) Chain: C
Molecule details ›
Chain: C
Length: 98 nucleotides
Theoretical weight: 31.72 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.43Å
Relevant EMDB volumes: EMD-39191
Expression system: Escherichia coli